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The Opera Phenix™ High Content Screening System is the premier confocal solution for today's most demanding high content applications. Drawing on over a decade of experience with the industry-leading Opera® High Content Screening System, the Opera Phenix is designed for high-throughput, phenotypic screening and assays involving complex disease models, such as live cells, primary cells and microtissues.
The Opera Phenix HCS system's innovative optical design lets you generate richer information through extremely sensitive confocal imaging and at higher throughput than ever through simultaneous acquisition – without the issue of crosstalk.
Watch the video to learn more about the Opera Phenix HCS System.
Speed and Sensitivity – No Compromise
Proprietary Synchrony™ Optics combine a microlens enhanced Nipkow spinning disk with dual view confocal optics to separate fluorescence excitation and emission during simultaneous acquisition minimizing spectral crosstalk – for more speed and more sensitivity.
Up to four large format sCMOS cameras with simultaneous acquisition in up to four channels, so you can generate images of exceptional quality - faster than ever
Custom-designed high NA water immersion objectives to capture more photons and provide high image resolution even in thick samples
Large field of view – 290% larger than the original Opera HCS system to acquire more cells in a single image for robust assay statistics
State-of-the-art sCMOS image sensors deliver low signal-to-background noise, a wide dynamic range and high resolution – ideal for sensitive and quantitative measurements at short exposure times
Spinning microlens disk with increased pinhole-to-pinhole distance to further reduce out-of-focus noise in thick samples such as microtissues
Spinning disk optics and precise synchronization of the excitation laser and camera to minimize phototoxicity and bleaching for live cell assays
Widefield or confocal imaging modes depending on your assay requirements
Fast laser-based autofocus to ensure sharp confocal images from every field
Modular design to adapt the system to your changing application needs – choose from single, dual or four camera configuration with four solid state lasers or a five laser system optimized for FRET applications
Watch the short webinar to learn about the advantages of using the Opera Phenix system for imaging 3D samples.
Opera Phenix 2
With the Opera Phenix HCS System you can generate high resolution images in up to four colors at ultra-high throughput.
It All Comes Together in Harmony – Acquisition to Analysis Made Easy
Harmony® High Content Imaging and Analysis Software makes it easy to control every aspect of your experiment and to manage large quantities of data.
Intuitive workflow user-interface guides you through the entire process, from acquisition to analysis and evaluation
Templates for set-up of acquisition channels and parameters to get you productive right away
Ready-made solutions for common image analysis tasks so you can simply select and go
Image analysis building blocks that allow you to create, configure, and customize your own high content analysis applications
Advanced analysis features such as texture and STAR morphology analysis for detailed descriptions of cellular morphology and robust differentiation of cellular phenotypes
Data management for automatic storage of analysis results and metadata, such as assay layout, instrument, settings, plus user-defined keywords, and annotations
Harmony
The Harmony software has an intuitive user-interface that guides you through the whole process from acquisition to analysis and evaluation.
Your Total Solution for High Content Screening
Get better results from your Opera Phenix system with our microplates for high content screening, such as the CellCarrier™ Ultra 384-well microplates designed for optimal performance in high content imaging applications
Improve throughput and productivity, and reduce variability and reagent costs by automating your Opera Phenix system, for example with the cell::explorer™ automated workstation
Export your results automatically into the Columbus™ Image Data Storage and Analysis System, so you can access, reanalyze, store, and share image data from Opera Phenix and other HCS systems across your organization
NEW! Export your results to High Content Profiler™, powered by TIBCO Spotfire®, and perform screening data analysis and validation, QC analyses, calculate reliable normalization, perform multivariate hit stratification and drug response profiling
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